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RNAscope原位杂交技术对复杂组织进行空间表达分析

直播日期 2020/06/30 16:30

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RNAscope原位杂交技术对复杂组织进行空间表达分析

RNAscope和BaseScope原位杂交(ISH)广泛应用于人类样本库和临床科研以及临床前动物模型等组织中的高分辨率目标RNA表达分析。ACD的RNA-ISH检测在临床实验研究中是有效的,能够在复杂的组织微环境中进行定量的、细胞特异性的表达分析。
RNAscope和相关ISH技术的应用进展包括:
- 固定组织中RNA的单分子检测
- 空间、多重RNA-ISH用于RNAseq转录组学的验证
- SARS-CoV-2及其他病毒病原体检测
- 实体瘤组织中CAR-T细胞的检测
- AAV基因治疗的生物分布

RNAscope and BaseScope in situ hybridization (ISH) platforms are utilized worldwide for high resolution tissue-based target expression analysis in human archival and clinical biopsies, as well as in preclinical animal models. ACD’s RNA ISH assays are clinically validated and enable quantitative, cell-specific expression analysis within complex tissue microenvironments.
Advances in RNAscope and related ISH technologies will be discussed:
- Single-molecule detection of RNA in fixed tissues
- Spatial, multiplexed RNA ISH for validation of RNAseq transcriptomics
- SARS-CoV-2 and other viral pathogen assays
- CAR-T cell detection in solid tumor tissues
- AAV gene therapy biodistribution analysis

嘉宾简介

Christopher Bunker
Ph.D.,MBA,Sr. Director
快三网上购买(Advanced Cell Diagnostics)

Dr。 Christopher Bunker is the Sr。 Director of Business Development at Advanced Cell Diagnostics。 Dr。 Bunker did his doctoral research in Biological Chemistry at Harvard University and a Leukemia Society of America postdoctoral fellowship in molecular genetics at University of California, San Francisco。 He was a senior scientist at Genome Therapeutics before joining Cell Signaling Technology at its inception in 2000。 Dr。 Bunker received an M。B。A from Boston University, and then joined Advanced Cell Diagnostics, where he has led its assay services business for the past nine years。

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